dna prepared from the map reference strain cc 19698 Search Results


positive dna controls  (ATCC)
93
ATCC positive dna controls
Positive Dna Controls, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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m avium subsp paratuberculosis strains  (ATCC)
92
ATCC m avium subsp paratuberculosis strains
FIG. 1. Multiple nucleotide sequence alignment of M. <t>avium</t> <t>subsp.</t> avium (Maa), M. avium subsp. <t>paratuberculosis</t> (Map) and M. bovis (Mbo) us-p34 genes. Gaps between sequences are indicated by dots. Vertical bars indicate identity across sequences. The start codon (ATG) for the p34 open reading frame is shown in bold. Primer positions are indicated by boxes. The horizontal arrows indicate directions of transcription. The Hpy188I restriction site is indicated by a boldly outlined box. The single nucleotide substitution between M. avium subsp. paratuberculosis and M. avium subsp. avium within the restriction site is highlighted with an arrow and a bold nucleotide. Nucleotides shown in bold italics in the myc3 sequence were degenerated in the corresponding primer (see Table 1).
M Avium Subsp Paratuberculosis Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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m avium subsp paratuberculosis atcc 19698 genomic dna  (ATCC)
96
ATCC m avium subsp paratuberculosis atcc 19698 genomic dna
FIG. 1. Multiple nucleotide sequence alignment of M. <t>avium</t> <t>subsp.</t> avium (Maa), M. avium subsp. <t>paratuberculosis</t> (Map) and M. bovis (Mbo) us-p34 genes. Gaps between sequences are indicated by dots. Vertical bars indicate identity across sequences. The start codon (ATG) for the p34 open reading frame is shown in bold. Primer positions are indicated by boxes. The horizontal arrows indicate directions of transcription. The Hpy188I restriction site is indicated by a boldly outlined box. The single nucleotide substitution between M. avium subsp. paratuberculosis and M. avium subsp. avium within the restriction site is highlighted with an arrow and a bold nucleotide. Nucleotides shown in bold italics in the myc3 sequence were degenerated in the corresponding primer (see Table 1).
M Avium Subsp Paratuberculosis Atcc 19698 Genomic Dna, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human sun2 gene orf cdna clone in cloning vector  (Sino Biological)
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Full length Clone DNA of Human Sad1 and UNC84 domain containing 2
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FIG. 1. Multiple nucleotide sequence alignment of M. avium subsp. avium (Maa), M. avium subsp. paratuberculosis (Map) and M. bovis (Mbo) us-p34 genes. Gaps between sequences are indicated by dots. Vertical bars indicate identity across sequences. The start codon (ATG) for the p34 open reading frame is shown in bold. Primer positions are indicated by boxes. The horizontal arrows indicate directions of transcription. The Hpy188I restriction site is indicated by a boldly outlined box. The single nucleotide substitution between M. avium subsp. paratuberculosis and M. avium subsp. avium within the restriction site is highlighted with an arrow and a bold nucleotide. Nucleotides shown in bold italics in the myc3 sequence were degenerated in the corresponding primer (see Table 1).

Journal: Journal of Clinical Microbiology

Article Title: Definitive Differentiation between Single and Mixed Mycobacterial Infections in Red Deer ( Cervus elaphus ) by a Combination of Duplex Amplification of p34 and f57 Sequences and Hpy188I Enzymatic Restriction of Duplex Amplicons

doi: 10.1128/jcm.43.9.4640-4648.2005

Figure Lengend Snippet: FIG. 1. Multiple nucleotide sequence alignment of M. avium subsp. avium (Maa), M. avium subsp. paratuberculosis (Map) and M. bovis (Mbo) us-p34 genes. Gaps between sequences are indicated by dots. Vertical bars indicate identity across sequences. The start codon (ATG) for the p34 open reading frame is shown in bold. Primer positions are indicated by boxes. The horizontal arrows indicate directions of transcription. The Hpy188I restriction site is indicated by a boldly outlined box. The single nucleotide substitution between M. avium subsp. paratuberculosis and M. avium subsp. avium within the restriction site is highlighted with an arrow and a bold nucleotide. Nucleotides shown in bold italics in the myc3 sequence were degenerated in the corresponding primer (see Table 1).

Article Snippet: Reference strains included two M. avium subsp. avium strains (ATCC 25291 and DSMZ 44156), three M. avium subsp. paratuberculosis strains (ATCC 19698, ATCC 43015, known as strain Linda [2], and the vaccinal strain 2E [33]), and one M. bovis strain (ATCC 19210).

Techniques: Sequencing

FIG. 2. Nucleotide sequence of M. avium subsp. paratuberculosis f57 segment (29a). Primer positions are indicated by boxes. The horizontal arrows indicate directions of transcription. Hpy188I restriction sites are shown in bold.

Journal: Journal of Clinical Microbiology

Article Title: Definitive Differentiation between Single and Mixed Mycobacterial Infections in Red Deer ( Cervus elaphus ) by a Combination of Duplex Amplification of p34 and f57 Sequences and Hpy188I Enzymatic Restriction of Duplex Amplicons

doi: 10.1128/jcm.43.9.4640-4648.2005

Figure Lengend Snippet: FIG. 2. Nucleotide sequence of M. avium subsp. paratuberculosis f57 segment (29a). Primer positions are indicated by boxes. The horizontal arrows indicate directions of transcription. Hpy188I restriction sites are shown in bold.

Article Snippet: Reference strains included two M. avium subsp. avium strains (ATCC 25291 and DSMZ 44156), three M. avium subsp. paratuberculosis strains (ATCC 19698, ATCC 43015, known as strain Linda [2], and the vaccinal strain 2E [33]), and one M. bovis strain (ATCC 19210).

Techniques: Sequencing

FIG. 3. Banding patterns of undigested us-p34–f57 amplicons. (A) Theoretical banding patterns before Hpy188I digestion (, Hpy188I restriction site). (B) Amplified DNA fragments from M. bovis (lane 3), M. avium subsp. paratuberculosis (lane 4), M. avium subsp. avium (lane 5), a mixture of M. avium subsp. avium and M. bovis (lane 6), a mixture of M. avium subsp. avium and M. avium subsp. paratuberculosis (lane 7), and a mixture of M. avium subsp. avium, M. avium subsp. paratuberculosis, and M. bovis (lane 8) were separated by electrophoresis in a 2% agarose gel. The lengths (in base pairs) of the amplified fragments and of the 123-bp DNA ladder (lane 1) and 50-bp DNA ladder (lane 2) (molecular weight markers) are indicated on the right and left sides of the gel, respectively. Hatched boxes represent the p34 gene.

Journal: Journal of Clinical Microbiology

Article Title: Definitive Differentiation between Single and Mixed Mycobacterial Infections in Red Deer ( Cervus elaphus ) by a Combination of Duplex Amplification of p34 and f57 Sequences and Hpy188I Enzymatic Restriction of Duplex Amplicons

doi: 10.1128/jcm.43.9.4640-4648.2005

Figure Lengend Snippet: FIG. 3. Banding patterns of undigested us-p34–f57 amplicons. (A) Theoretical banding patterns before Hpy188I digestion (, Hpy188I restriction site). (B) Amplified DNA fragments from M. bovis (lane 3), M. avium subsp. paratuberculosis (lane 4), M. avium subsp. avium (lane 5), a mixture of M. avium subsp. avium and M. bovis (lane 6), a mixture of M. avium subsp. avium and M. avium subsp. paratuberculosis (lane 7), and a mixture of M. avium subsp. avium, M. avium subsp. paratuberculosis, and M. bovis (lane 8) were separated by electrophoresis in a 2% agarose gel. The lengths (in base pairs) of the amplified fragments and of the 123-bp DNA ladder (lane 1) and 50-bp DNA ladder (lane 2) (molecular weight markers) are indicated on the right and left sides of the gel, respectively. Hatched boxes represent the p34 gene.

Article Snippet: Reference strains included two M. avium subsp. avium strains (ATCC 25291 and DSMZ 44156), three M. avium subsp. paratuberculosis strains (ATCC 19698, ATCC 43015, known as strain Linda [2], and the vaccinal strain 2E [33]), and one M. bovis strain (ATCC 19210).

Techniques: Electrophoresis, Agarose Gel Electrophoresis, Molecular Weight

FIG. 4. Hpy188I digestion profiles of duplex us-p34–f57 amplicons. (A) Theoretical banding patterns after Hpy188I digestion. (B) Banding patterns of M. bovis (lane 3), M. avium subsp. paratuberculosis (lane 4), M. avium subsp. avium (lane 5), a mixture of M. avium subsp. avium and M. bovis (lane 6), a mixture of M. avium subsp. avium and M. avium subsp. paratuberculosis (lane 7), and a mixture of M. avium subsp. avium, M. avium subsp. paratuberculosis, and M. bovis (lane 8). The lengths (in base pairs) of the amplified fragments and of the 123-bp DNA ladder (lane 1) and the 50-bp DNA ladder (lane 2) (molecular weight markers) are indicated on the right and left sides of the gel, respectively.

Journal: Journal of Clinical Microbiology

Article Title: Definitive Differentiation between Single and Mixed Mycobacterial Infections in Red Deer ( Cervus elaphus ) by a Combination of Duplex Amplification of p34 and f57 Sequences and Hpy188I Enzymatic Restriction of Duplex Amplicons

doi: 10.1128/jcm.43.9.4640-4648.2005

Figure Lengend Snippet: FIG. 4. Hpy188I digestion profiles of duplex us-p34–f57 amplicons. (A) Theoretical banding patterns after Hpy188I digestion. (B) Banding patterns of M. bovis (lane 3), M. avium subsp. paratuberculosis (lane 4), M. avium subsp. avium (lane 5), a mixture of M. avium subsp. avium and M. bovis (lane 6), a mixture of M. avium subsp. avium and M. avium subsp. paratuberculosis (lane 7), and a mixture of M. avium subsp. avium, M. avium subsp. paratuberculosis, and M. bovis (lane 8). The lengths (in base pairs) of the amplified fragments and of the 123-bp DNA ladder (lane 1) and the 50-bp DNA ladder (lane 2) (molecular weight markers) are indicated on the right and left sides of the gel, respectively.

Article Snippet: Reference strains included two M. avium subsp. avium strains (ATCC 25291 and DSMZ 44156), three M. avium subsp. paratuberculosis strains (ATCC 19698, ATCC 43015, known as strain Linda [2], and the vaccinal strain 2E [33]), and one M. bovis strain (ATCC 19210).

Techniques: Molecular Weight